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If producing v2 sample sheets for devices apart from those outlined, affirm the final sample sheet has the i5 index in the right orientation as explained over.
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The MiniSeq Fast built a recipe improve that takes advantage of the grafted oligo for the i5 index priming, so it truly is read during the ahead orientation much like a MiSeq.
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Streamlined RNA-Seq Answer for distinct and complete Evaluation across the coding and non-coding transcriptome with Outstanding study adaptability.
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When employing BaseSpace Sequence Hub PrepTab, i5 sequences must be entered into these application during the ahead orientation in all situations and for all instrument sorts.
For runs uploaded manually (i.e., an Illumina Resource just isn't getting used to make the sample sheet), the index orientation is determined by which instrument you are working with. Remember to begin to see the Index Orientation Table for info on website the best way to enter the i5 sequence based on the instrument and program utilised.